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Published by New Mexico Tech Department of Biology
Contact Webmaster:  biology@nmt.edu
Thomas L. Kieft
Professor of Biology
New Mexico Tech
801 Leroy Pl.,
Socorro, NM 87801
Phone: (575) 835-5321
FAX: (575) 835-5668
Office: Jones Annex 2##
E-mail: tkieft@nmt.edu





Peer-Reviewed Publications

Lau, M.C.Y., T.L. Kieft, O. Kuloyo, B. Linage-Alvarez, E. van Heerden, M.R. Lindsay, C. Magnabosco, W. Wang,
J.B. Wiggins, L. Guo, D.H. Perlman, S.Kyin, H.H. Shwe, R.L. Harris, Y. Oh, M.J. Yi, R. Purtschert, G.F. Slater, S.
Ono, S. Wei, L. Li, B. Sherwood Lollar, and T.C. Onstott.  2016.  Oligotrophic deep subsurface community dependent
on syntrophy is dominated by sulfur-driven autotrophic denitrifiers. Proceedings of the National Academy of Sciences
USA 113:E7927-E7936. doi: 10.1073/pnas.1612244113.

Simkus, D.N., G.F. Slater, B. Sherwood Lollar, K.Wilkie,, T.L. Kieft, C. Magnabosco, M.C.Y. Lau, M.J. Pullin, S.B.
Hendrickson, K.E.Wommack, E.G. Sakowski, E.van Heerden, O. Kuloyo, B. Linage, G. Borgonie, T.C. Onstott.
Variations in microbial carbon sources and cycling in the deep continental subsurface. Geochimica Cosmochimica
Acta (in press).

Magnabosco, C., K. Ryan, M.C.Y. Lau, K. Olukayode, B. Sherwood Lollar, T.L. Kieft, E. van Heerden, and T.C.
Onstott.  A metagenomic window into carbon metabolism at 3 km depth in Precambrian continental crust.  ISME
Journal doi:10.1038/ismej.2015.150 (in press).

Kieft, T.L., and K.A. Simmons. 2015. Allometry of animal-microbe interactions and global census of animal-
associated microbes. Proceedings of the Royal Society of London B 282 20150702; doi: 10.1098/rspb.2015.0702.
Published  24 June 2015.

Kieft, T.L., T.C. Onstott, L. Ahonen, V. Aloisi, F.S. Colwell, B. Engelen, S. Fendrihan, E. Gaidos, U. Harms, I Head,
J. Kallmeyer, B. Kiel Reese, L.-H. Lin, P.E. Long, H. Mills, D.P. Moser, P. Sar, D. Schulze-Makuch, H. Stan-Lotter,
D. Wagner, P.-L. Wang, F. Westall, and M.J. Wilkins.  2015. Workshop to develop deep life continental scientific
drilling projects. Scientific Drilling 19, 43-53

Labonté, J.M., E.K. Field, M. Lau, D. Chivian, E. Van Heerden, K.E. Wommack, T.L. Kieft, T.C. Onstott, R.
Stepanauskas. 2015. Single cell genomics indicates horizontal gene transfer and viral infections in a deep subsurface
Firmicutes population. Frontiers in Microbiology 6:349, pp. 1-11, doi: 10.3389/fmicb.2015.00349.

Magnabosco, C., M. Tekere, M.C.Y. Lau, B. Linage, O. Kuloyo, M. Erasmus, E. Cason, E. van Heerden, G.
Borgonie, T.L. Kieft, T.C. Onstott.  2014. Comparisons of the composition and biogeographic distribution of the
bacterial communities occupying South African thermal springs with those inhabiting deep subsurface fracture water.
Frontiers in Microbiology Vol. 5, article 679, pp. 1-17,  doi:  10.3389/fmicb.2014.00679

Lau, M.C.Y., C. Cameron, C. Magnabosco, C .T. Brown, F.Schilkey, S. Grim, S. Hendrickson, M. Pullin, B.
Sherwood Lollar, E. van Heerden, T.L. Kieft, T.C. Onstott. 2014. Phylogeny and phylogeography of functional genes
shared among seven terrestrial subsurface metagenomes reveal N-cycling and microbial evolutionary relationships.
Frontiers in Microbiology Vol. 5, article 531, 1-17.

Rummel, J.D., D.W. Beaty, M.A. Jones, C. Bakermans, N.G. Barlow, P. Boston, V. Chevrier, B.C. Clark, J-P. de
Vera, R.V. Gough, J.E. Hallsworth, J.W. Head, V.J. Hipkin, T.L. Kieft, A.S. McEwen, M.T. Mellon, J. Mikucki,
W.L. Nicholson, C.R. Omelon, R. Peterson, E. Roden, B. Sherwood Lollar, K.L. Tanaka, D. Viola, and J.J. Wray, A
New Analysis of Mars 'Special Regions': Findings of the Second MEPAG Special Regions Science Analysis Group
(SR-SAG2).  Astrobiology 14(11): 887-968.

Wilkins M.J., R. Daly, P.J. Mouser, R. Trexler, K.C Wrighton, S. Sharma, D.R. Cole, J.F. Biddle, E. Denis, J.K.
Fredrickson, T.L. Kieft, T.C Onstott, L. Petersen, S.M. Pfiffner, T. J. Phelps, M.O. Schrenk.  2014. Trends and
Future Challenges in Sampling the Deep Terrestrial Biosphere.  Frontiers in Microbiology 5:481.

Onstott, T.C., C. Magnabosco, A.D. Aubrey, A.S. Burton, J.P. Dworkin, J.E. Elsila, S. Grunsfeld, B.H. Cao, J.E.
Hein, D.P. Glavin, T.L. Kieft, B.J. Silver, T.J. Phelps, E. van Heerden, D.J. Opperman, J.L. Bada. 2014. Does
Aspartic Acid Racemization Constrain the Depth Limit of the Subsurface Biosphere?  Geobiology 12:1-19.

Davidson, M.M., B.J. Silver, T.C. Onstott, D.P. Moser, T.M. Gihring, L.M. Pratt, E.A. Boice, B. Sherwood Lollar, J.
Lippmann-Pipke, S.M. Pfiffner, T.L. Kieft, W. Seymore, and C. Ralston.  2011. Capture of Planktonic Microbial
Diversity in Fractures by Long-Term Monitoring of Flowing Boreholes, Evander Basin, South. Geomicrobiology
Journal 28:275-300.

Kminek, G., J.D. Rummel, C.S. Cockell , R. Atlas, N. Barlow, D. Beaty, W. Boynton, M. Carr, S. Clifford, C.A.
Conley, A.F. Davila, A. Debus, P. Doran, M. Hecht, J. Heldmann, J. Helbert, V. Hipkin, G. Horneck, T.L. Kieft, G.
Klingelhoefer, M. Meyer, H. Newsom, G.G. Ori, J. Parnell, D. Prieur, F. Raulin, D. Schulze- Makuch, J.A. Spry, P.E.
Stabekis, E. Stackebrandt, J. Vago, M. Viso, M. Voytek, L. Wells, F. Westall. 2010. Report of the COSPAR mars
special regions colloquium. Advances in Space Research 46:811-829.

Tang, H., P. Zhang, T.L. Kieft, S.J. Ryan, S.M. Baker, W.P. Wiesmann, S. Rogelj. 2010. Antibacterial action of a
novel functionalized chitosan-arginine against gram-negative bacteria. Acta Biomaterialia 6:2562-2571.

Silver, B.J., T.C. Onstott, G. Rose, L.-H., Lin, C. Ralston, B. Sherwood-Lollar, S. M. Pfiffner, T. L. Kieft, and S.
McCuddy. 2010. In situ cultivation of subsurface microorganisms in a deep mafic sill: implications for SLiMEs.
Geomicrobiology Journal 27:329-348.

Sahl, J.W., R. Schmidt, E.D. Swanner, K.W. Mandernack, A.S. Templeton, T.L. Kieft, R.L. Smith, W.E. Sanford,
R.L. Calllaghan, J.B. Mitton, and J.R. Spear. 2008. Subsurface microbial diversity in deep- granitic-fracture water in
Colorado. Applied and Environmental Microbiology 74:143-152.   

MEPAG Special Regions Science Analysis Group (Beaty D., K. Buxbaum, M. Meyer, N. Barlow, W. Boynton, B.
Clark, J. Deming, P. Doran, K. Edgett, S. Hancock, J. Head, M. Hecht, V. Hipkin, T. Kieft, R. Mancinelli, E.
McDonald, C. McKay, M. Mellon, H. Newsom, G. Ori, D. Paige, A. Schuerger, M. Sogin, J. A. Spry, A. Steele, K.
Tanaka, M. Voytek). 2006. Findings of the Mars Special Regions Science Analysis Group. Astrobiology 6:677-732.

Kieft, T. L., and D. P. Moser. 2006. Special issue on geomicrobiology of the deep biosphere - Preface. 
Geomicrobiology Journal 23:34

Gihring, T., Moser DP, Lin L-H, Davidson M., Onstott TC, Morgan L, Milleson M., Kieft TL, Trimarco E, Balkwill
DL, Dollhopf ME.  The distribution of microbial taxa in the subsurface water of the Kalahari Shield, South Africa.
Geomicrobiology Journal.

Onstott, T.C., L.-H. Lin, M. Davidson, B. Mislowac, M. Borcsik, J. Hall, G. Slater, J. Ward, B. Sherwood Lollar, J.
Lippmann-Pipke, E. Boice, L. Pratt, B. S. Pfiffner, D. Moser, T. Gihring, T.L. Kieft, T.J. Phelps, E van Heerden, D.
Litthauer, M. DeFlaun, and R. Rothmel. The origin and age of biogeochemical trends in deep fracture water of the
Witwatersrand basin, South Africa. Geomicrobiology Journal.

Shen, H., S. Rogelj, and T. Kieft. Sensitive, real-time PCR detects low-levels of contamination by Legionella
pneumophila in commercial reagents. Molecular and Cellular Probes20:147-153.   

Allen, R. C., S. Rogelj, and T. L. Kieft. 2006. An Immuno-PCR method for detecting Bacillus thuringiensis Cry1Ac
toxin. Journal of Immunological Methods 308:109-115.

Kieft, T.L., S.M. McCuddy, T.C. Onstott, L.-H. Lin, M. Davidson, B. Mislowac, L. Pratt, E. Boice, B. Sherwood-
Lollar, L.-H. Lin, J. Lippmann, S.M. Pfiffner, T.J. Phelps, T. Gihring, D. Moser, and A. van Heerden. 2005.
Geochemically generated, energy-rich substrates and indigenous microorganisms in deep, ancient groundwater.
Geomicrobiology Journal 22:325-335.   

Fu, Z., S. Rogelj, and T. L. Kieft. Detection of Escherichia coli 0157:H7 by immuno-magnetic separation and real-time
PCR. International Journal of Food Microbiology 99:47-57.

Rutz, B. and T. L. Kieft. 2004. Phylogenetic characterization of dwarf archaea and bacteria from a semiarid soil. Soil
Biology and Biochemistry 36:825-833.

Balkwill, D. L., T. L. Kieft, T. Tsukuda, H. M. Kostandarithes, T. C. Onstott, S. Macnaughton, J. Bownas, and J.K.
Fredrickson. 2004. Identification of iron-reducing Thermus strains as T. scotoductus. Extremophiles 8:37-44.

Lehman, R. M., S. P. O'Connell, A. Banta, J. K. Fredrickson, A.-L. Reysenbach, T. L. Kieft, and F. S. Colwell. 2004.
Microbiological comparison of core and groundwater samples collected from a fractured basalt aquifer with that of
dialysis chambers incubated in situ. Geomicrobiology Journal 21:169-182.

Onstott, T. C., D. P. Moser, S. M. Pfiffner, J. K. Fredrickson, F.J. Brockman, T. J. Phelps, D. C. White, A. Peacock,
D. Balkwill, R. Hoover, L. R. Krumholz, M. Borscik, T. L. Kieft, and R. Wilson. 2003. Indigenous and introduced
microorganisms in rock samples from a deep gold mine. Environ. Microbiol. 5:1168-1191.

Liang, H., S. E. Cordova, T. L. Kieft, and S. Rogelj. 2003. Development of an immuno-PCR assay to detect Group A
Streptococcus. J. Immunol. Methods 279:101-10.

Oliver, D. S., F. J. Brockman, R. S. Bowman, and T. L. Kieft. 2003. Microbial Reduction of Hexavalent Chromium
under Vadose Zone Conditions. Journal of Environmental Quality 32:317-324.

Ayarbe, J. P., and T. L. Kieft. 2000. Mammal mounds stimulate microbial activity in a semiarid shrubland. Ecology
81:1150-1154.

Allen. C. C., F. G. Albert, H. S. Chafetz, J. Combie, C. R. Graham, T. L. Kieft, S. J. Kivett, D. S. McKay, A. Steele,
A. E. Taunton, M. R. Taylor, K. L. Thomas-Keprta, and F. Westall. 2000. Physical biomarkers in carbonate thermal
springs: implications for Mars. Icarus 147:49-67.

Kieft, T. L., J. K. Fredrickson, T. C. Onstott, Y. A. Gorby, H. M. Kostandarithes, T. J. Bailey, D. W. Kennedy, S.
W. Li, A. Plymale, C. M. Spadoni, and M. S. Gray. 1999. Dissimilatory reduction of Fe(III) and other electron
acceptors by a Thermus isolate. Applied and Environmental Microbiology 65:1214-1221.

Walvoord, M. A., F. Phillips, T. L. Kieft, J. K. Fredrickson, P. Pegram, and M. Person. 1999. Groundwater flow and
geochemistry in the southeastern San Juan Basin: implications for microbial transport and activity. Water Resources
Research 35:1409-1424.

Kieft, T. L., E. M. Murphy, D. L. Haldeman, P. S. Amy, B. N. Bjornstadt, E. V. McDonald, D. B. Ringelberg, D. C.
White, J. O. Stair, R. P. Griffiths, T. C. Gsell, W. E. Holben, and D. R. Boone. 1998. Microbial transport, survival, and
succession in a sequence of buried sediments. Microbial Ecology 36:336-348.

Onstott, T. C., T. J. Phelps, F. S. Colwell, D. Ringelberg, D. C. White, D. R. Boone, J. P. McKinley, T. O. Stevens,
P. E. Long, D. L. Balkwill, T. Griffin, and T. Kieft. 1998. Observations pertaining to the origin and ecology of
microorganisms recovered from the deep subsurface of Taylorsville Basin, Virginia. Geomicrobiology Journal 15:353-
385.

Kieft, T. L., C. S. White, S. R. Loftin, R. Aguilar, J. R. Craig, and J. A. Skaar. 1998. Temporal dynamics in soil
carbon and nitrogen resources at a grassland-shrubland ecotone. Ecology 79:671-683.

Brockman, F. J., S. W. Li, J. K. Fredrickson, D. B. Ringelberg, T. L. Kieft, C. M. Spadoni, D. C. White, and J. P.
McKinley. 1998. Post-sampling changes in microbial community composition and activity in a subsurface paleosol.
Microbial Ecology 36:152-164.

Kieft, T. L., W. P. Kovacik, Jr., D. B. Ringelberg, D. C. White, D. L. Haldeman, P. S. Amy, and L. E. Hersman.
1997. Factors limiting to microbial growth and activity at a proposed high-level nuclear repository, Yucca Mountain,
Nevada. Applied and Environmental Microbiology 63:3128-3133.

Kieft, T. L., E. Wilch, K. O'Connor, D. B. Ringelberg, and D. C. White. 1997. Survival and phospholipid fatty acid
profiles of surface and subsurface bacteria in natural sediment microcosms. Applied and Environmental Microbiology
63:1531-1542.

Kieft, T. L., J. K. Fredrickson, J. P. McKinley, B. N. Bjornstad, S. A. Rawson, T. J. Phelps, F. J. Brockman, and S.
M. Pfiffner. 1995. Microbiological Comparisons within and across Contiguous Lacustrine, Paleosol, and Fluvial
Subsurface Sediments. Applied and Environmental Microbiology 61:749-757.

Kieft, T. L., D. B. Ringelberg and D. C. White. 1994. Changes in ester-linked phospholipid fatty acid profiles of
subsurface bacteria during starvation and desiccation in a porous medium. Applied and Environmental Microbiology
60:3292-3299.

Kieft, T. L. 1994. Grazing and plant canopy effects on semiarid soil microbial biomass and respiration. Biology and
Fertility of Soils 18:155-162.

Brown, R. L., R. S. Bowman and T. L. Kieft. 1994. Microbial effects on nickel and cadmium sorption and transport in
volcanic tuff. Journal of Environmental Quality 28:723-729.

Fredrickson, J. K., F. J. Brockman, B. N. Bjornstad, P. E. Long, S. W. Li, J. P. McKinley, J. L. Conca, T. L. Kieft
and D. L. Balkwill. 1994. Microbiological characteristics of pristine and contaminated deep vadose sediments from an
arid region. Geomicrobiology Journal 11:95-107.

Wan, J., J. L. Wilson and T. L. Kieft. 1994. Influence of the gas-water interface on transport of microorganisms
through unsaturated porous media. Applied and Environmental Microbiology 60:509-516.

Kieft, T. L., P. S. Amy, B. N. Bjornstad, F. J. Brockman, J. K. Fredrickson and L. L. Rosacker. 1993. Microbial
Abundance and Activities in Relation to Water Potential in the Vadose Zones of Arid and Semiarid Sites. Microbial
Ecology 26:59-78.

Ashworth, E. N. and T. L. Kieft. 1992. Measurement of ice nucleation in lichens using thermal analysis. Cryobiology
29:400-406.

Kieft, T. L. and T. Ruscetti. 1992. Molecular sizes

Book Reviews

Kieft, T. L. 1995. Review of Biodegradation and Bioremediation, by Martin Alexander. Journal of Environmental
Quality 24 (3).

Kieft, T. L. 1991. "For ecologists and 'E. coli-gists'," [Review of Bacterial Genetics in Natural Environments by J. C.
Fry and M. J. Day]. Trends in Genetics 7:236-237.

Patent

Kieft, T. L.  1992.  Increasing nucleation activity with lichens and fungi. Patent N

Book Chapters

Kieft, T.L. Microbiology of the deep continental biosphere.  Ch. 6 In:Volume 1 of Advances in Environmental
Microbiology "Their World: a diversity of environments" (editor: Christon J. Hurst), Springer, New York. (in press)

Kieft, T.L. Sampling the subsurface. In: Hydrocarbons and Lipid Microbiology (editors: Terence J. McGenity,
Kenneth M. Timmis, Balbina Nogales) Springer Protocols Handbooks.  Springer-Verlag, Heidelberg. (in press)

Kieft, T.L. 2010. Sampling the deep sub-surface using drilling and coring techniques. pp. 3427-3441, In: Microbiology
of Hydrocarbons and Lipids.  K.N. Timmis (Ed.), Springer Verlag, Berlin.

Kieft, T. L., Phelps, T. J., and J. K. Fredrickson.  2007. Drilling, coring, and sampling subsurface environments. pp.
799-817, In: Manual of Environmental Microbiology, Third Edition. Hurst, C.J. (Ed.), ASM Press, Washington, DC.

Horsfield, B., Kieft, T. L., Amann, H., Franks, S. G., Kallmeyer, J., Mangelsdorf, K., Parkes, R. J., Wagner, D.,
Wilkes, H., Zink, K.-G. 2007. The GeoBiosphere.  pp. 163-211. In: U. Harms, C. Koeberl and M. D. Zoback (Eds.)
Continental Scientific Drilling. A Decade of Progress, and Challenges for the Future. Springer Verlag, Berlin.

Kieft, T.L. 2002. Hot Desert Soil Communities. pp. 1576-1586. In: Encyclopedia of Environmental Microbiology, G.
Bitton (Ed.)., John Wiley, NY.

Kieft, T.L. 2002. Microbial Starvation Survival in Subsurface Environments. pp. 2019-2028. In: Encyclopedia of
Environmental Microbiology, G. Bitton (Ed.) John Wiley, NY.

Brockman, F.J., S.N. Bradley, and T. L. Kieft. 2002. Vadose Zone Microbiology. pp. 3236-3246. In: Encyclopedia of
Environmental Microbiology, G. Bitton (Ed.) John Wiley, NY.

Kieft, T. L., and F. J. Brockman. Vadose zone microbiology. 2001. pp. 141-169. In: Subsurface Microbiology and
Biogeochemistry. J. K. Fredrickson and M. Fletcher (Eds.), John Wiley & Sons, New York.

Kieft, T. L. 2000. Size matters: dwarf cells in soil and subsurface terrestrial environments. Ch. 3, pp. 19-46. In: Non-
culturable Microorganisms in the Environment. R. R. Colwell and D. J. Grimes (Eds.), American Society for
Microbiology, Washington, DC.

Onstott, T. C., T. J. Phelps, T. L. Kieft, F. S. Colwell, D. L. Balkwill., J. K. Fredrickson, and F. J. Brockman. 1999. A
global perspective on the microbial abundance and activity in the deep subsurface. Ch. 38 pp. 489-500. In: Enigmatic
Microorganisms and Life in Extreme Environments. J. Seckbach (Ed.), Kluwer Publications.

Kieft, T. L., and T. J. Phelps. 1997. Life in the slow lane: Activities of microorganisms in the subsurface. Ch. 12. pp.
137-163. In: The Microbiology of the Terrestrial Subsurface. P. S. Amy and D. L. Haldeman (Eds.), CRC Press,
Boca Raton.

Ashworth, E. N., and T. L. Kieft. 1995. Ice nucleation activity associated with plants and fungi. pp. 137-162. In:
Biological Ice

Nucleation and its Applications. R. E. Lee, G. Warren, and L. V. Gusta, (Eds.), Amer. Phytopathological Society, St.
Paul, MN.

Kieft, T. L. 1991. Soil microbiology in reclamation of arid and semi-arid lands. pp. 209-256. In: Semiarid Lands and
Deserts: Soil Resource and Reclamation. J. Skugins (Ed.), Marcel Dekker, New York.Kieft, T.L. 2002.

Abstracts, Posters, Presentations

Pfiffner, S. M., K. L. Davis, T. J. Phelps, T. L. Kieft, T. M. Gihring, T. C. Onstott, B. Nthangeru, L. Piater, and E.
van Heerden. Research Experience for Undergraduates: An International Program Enhancing Interdisciplinary
Learning. American Geophysical Union Meeting, San Francisco, December, 2004.

Kieft, T. L. Life in the Deep Biosphere. Physics Department, University of Minnesota, December 4, 2004 (Invited
talk).

Kieft, T. L. Microbial Life in the Deepest Regions of the Earth's Biosphere. Session Organizer and Convener. Amer.
Soc. Microbiol. General Meeting, New Orleans, LA, May 2004.

Wanger, G., T. L. Kieft, and G. Southam. Burrowing Behavior Exhibited by a Thermus sp. utilizing ferric hydroxides
as an electron acceptor. Amer. Soc. Microbiol. General Meeting, New Orleans, LA, May 2004.

Pfiffner, S. M., K. L. Davis, T. J. Phelps, T. L. Kieft, T. C. Onstott, and E.van Heerden. Use of Group Research
Teams to Enhance Interdisciplinary Learning During an International Research Experience for Undergraduates.
Amer. Soc. Microbiol. General Meeting, New Orleans, LA, May 2004.

Mislowack, B. J., T. Onstott, L.-H. Lin, G. Rose, C. Ralston, B. Sherwood-Lollar, S. M. Pfiffner, T. L. Kieft, S.
McCuddy. In Situ Cultivation of Deep Subsurface Microorganisms in a Mafic Sill: Implications for SLiME's. Amer.
Soc. Microbiol. General Meeting, New Orleans, LA, May 2004.

Moser, D. P., T. M. Gihring, J. K. Fredrickson, F. J. Brockman, T. C. Onstott, L.-H. Lin, B. Mislowak, M. Davidson,
D. L. Balkwill, M. E. Dollhopf, S. McCuddy, M. M. Milleson, T. L. Kieft, J. A. Hall. A Comprehensive Phylogenetic
Analysis of Microbial Communities from the Ultradeep Mines of South Africa. Amer. Soc. Microbiol. General
Meeting, New Orleans, LA, May 2004.

Liang, H., S. Cordova, R. Silva, Z. Fu, S. Rogelj, T. L. Kieft, D. Chandler, C. Bruckner-Lea, M. S. Bogucki, T.
Roberts, L. A. Pranger, and W. P. Weismann. Immunological and Nucleic-Acid Based Detection of Threat Agents:
From Lab Bench to Automated Platform. The International Symposium on Securing High Consequence Pathogens
and Toxins. Albuquerque, NM, February 1-6, 2004.

Kieft, T. L. Life in the Deep Biosphere. Biological Sciences Department, Idaho State University, Pocatello, ID,
December 4, 2003 (Invited talk).

Kieft, T. L. Life in the Deep Biosphere. Hydrology Program, New Mexico Tech, November 24, 2003 (Invited talk).

Silva, R. C. S. Rogelj, and T. L. Kieft. An immuno-PCR method for detecting Bacillusthuringiensis Cry1Ac toxin.
General Meeting, American Society for Microbiology, Washington, DC, May 2003.

Fu, Z., S. Rogelj, T. L. Kieft, Detection and Quantification of Escherichia coli O157:H7 by Immuno-magnetic
Separation and Real-time PCR, General Meeting, American Society for Microbiology, Washington, DC, May 2003.

Nolan, J. R., S. M. Pfiffner, K. L. Davis, T. J. Phelps, K. M. Coleman, T. L. Kieft, E. van Heerden, and D. Litthauer.
U.S./South African Undergraduate Education and Research Workshop. General Meeting, American Society for
Microbiology, Washington, DC, May 2003.

Shutthanandan, J., X. Yin, G. Drake, D.S. Oliver, T.L. Kieft, D.L. Balkwill, and F.J. Brockman. Differential
community structure in vadose zone microcosms supporting and not supporting chromium reduction during
bioremediation. General Meeting, American Society for Microbiology, Washington, DC, May 2003.

Kieft, T. L. Life in the Deep Biosphere, Biology Department, University of New Mexico, April 17, 2003 (Invited talk).

Huining Liang, Susan Cordova, Rebekah Silva, Zhu Fu, Snezna Rogelj, Thomas L. Kieft, Darrell Chandler, and Cindy
Bruckner-Lea, Mary S. Bogucki, Tara Roberts, L. Alex Pranger, and William P. Weismann. A flexible platform for
rapid, automated immunological and nucleic-acid based detection of biological threat agents. American Society for
Microbiology Biodefense Meeting, Baltimore, MD, March 2003. Kieft, T. L., and B. Rutz. Intrinsically and
pleomorphically dwarf bacteria and archaea in a semiarid soil. NASA Astrobiology Institute General Meeting, Tempe,
AZ, Feb.10-12, 2003.

Onstott, T.C. and the Witwatersrand Deep Microbiology Team. Nuclear-powered deep subsurface communities?
AAAS Annual Meeting, Denver, CO, February 13-18, 2003.

Kieft, T. L., Microbial Diversity in Deep Gold Mine Samples, Assessed by 16S rDNA Analyses. The Second US/SA
workshop on Biotechnological Applications of Deep Subsurface Microbial Investigations. December 16, 2002,
Pretoria, South Africa. (Invited talk).

Mislowack, B. J., Tullis C. Onstott, Li-hung Lin, Mark A. Davidson, Thomas J. Pray, Colin Ralston, Peter Roberts,
Tom L. Kieft, Tommy J. Phelps. In-situ Microbial Cultivation in a South African Gold Mine. American Geophysical
Union meeting, San Francisco, December 6-10, 2002.

Kieft, T. L. Life in the Deep Biosphere. University of Washington Astrobiology Program, Seattle, WA. October 8,
2002 (Invited talk).

Kieft, T. L. Life in the Deep Biosphere. University of the North, Pietersburg, South Africa, June 20, 2002 (Invited
talk).

Kieft, T. L. Life in the Deep Biosphere. New Mexico Museum of Space History, Alamogordo, NM, April 9, 2002
(Invited talk).

Liang, H., T. Kieft, and S. Rogelj. Development of an immuno-PCR assay to detect Group A Stretococcus. American
Society for Microbiology General Meeting, Salt Lake City, May 2002.

Shen, H., K. Ar., S. Rogelj, and T.L. Kieft. Quantitative detection of Legionella pneumophila in swamp coolers and
domestic water supplies. American Society for Microbiology General Meeting, Salt Lake City, May 2002.

Rutz, B. and T. L. Kieft. Intrinsically and pleomorphically dwarf bacteria and archaea in a semiarid soil. American
Society for Microbiology General Meeting, Salt Lake City, May 19-23, 2002.

Fu, Z., S. Rogelj, and T. Kieft. Detection and identification of pathogenic bacteria by cell adsorption and real-time
PCR. Rio Grande Branch American Society for Microbiology Annual Meeting, El Paso, TX, Feb. 2002.

Anbar, A. D., R. Buick, S. Mojzsis, A.J. Kaufman, T.L. Kieft, T.W. Lyons, and M. Humayun. 2001. The case for
scientific drilling of precambrian sedimentary sequences: a mission to early Earth. Geological Society of America
annual meeting, November 2001.

Anbar, A. D., R. Buick, S. Mojzsis, A.J. Kaufman, T.L. Kieft, T.W. Lyons, and M. Humayun. 2001. The case for
scientific drilling of precambrian sedimentary sequences: a mission to early Earth. American Geophysical Union
meeting, December 2001.

Brockman, F.J., D.O. Oliver, X. Yin, and T.L. Kieft. Effects of unsaturated flow on hexavalent chromium reduction
and 16S rRNA profiles. American Society for Microbiology Annual Meeting, Orlando, FL, May 2001.

Fuierer, A.M., R.S Bowman, M. McHale, B. Rutz, and T.L. Kieft. Biodegradation of toluene sorbed to surfactant-
modified zeolite. American Society for Microbiology Annual Meeting, Orlando, FL, May 2001.

Kieft, T. L. Mission to Early Earth: Sample Integrity and Geohydrology. Australian Astrobiology Workshop, July 2001.

McHale, M., T. L. Kieft, and N. W. Hinman. Archaeal and Bacterial diversity in a moderately acidic thermal iron
spring. NASA Astrobiology Institute Meeting, Washington, DC, April 2001.

McHale, M., T. L. Kieft, and N. W. Hinman. Archaeal and Bacterial diversity in a moderately acidic thermal iron
spring. Australian Astrobiology Workshop, July 2001.

McHale, M., T. L. Kieft, and N. W. Hinman. Archaeal and Bacterial diversity in a moderately acidic thermal iron
spring. International Symposium on Microbial Ecology, Amsterdam, August 2001.

Onstott, T.C., D. Moser, L. Lin, K. Takai, J. Fredrickson, F. Brockman, L. Pratt, B. Sherwood-Lollar, J. Hoek, G.
Omar, T.L. Kieft, D. Balkwill, S. Pfiffner, T. Phelps, J. Lippman, and M. Stute. Do superplumes select for
hyperthermophiles in the deep subsurface? Paper #7398. Symposium on Earth Systems Processes, Geological Society
of America and Geological Society of London, Edinburgh, Scotland, June 24-28, 2001.

Yin, X., D.O. Oliver, T.L. Kieft, and F.J. Brockman. Comparison of the microbial community under no flow,
unsaturated flow, and saturated flow in columns. American Society for Microbiology Annual Meeting, Miami, FL,
May 2001.

Kieft, T. L. The Deep Subsurface Biosphere. NASA Workshop on Extreme Environments, NASA-Ames, Moffett
Field, CA, July 24-25, 2001 (Invited).

Kieft, T. L. Mission to Early Earth: sample integrity and geohydrology. Australian Astrobiology Institute Workshop,
Sydney, Australia, July 12-13, 2001.

Kieft, T. L. Moisture and Nutrient Limitation of Microbial Activities in Arid and Semiarid Soils. U.S.-Egypt Microbial
Ecology Workshop, May 6-11, 2001 (Invited).

Kieft, T.L., Microbial activities in deep South African Au mines, University of Orange Free State, Bloomfontein, South
Africa, June 5, 2001 (Invited).

Fuierer, A. M., R. S. Bowman, and T. L. Kieft. 2001. Sorption and microbial degradation of toluene on a surfactant-
modified zeolite support. Proceedings of the Sixth International Symposium on In Situ and On-Site Bioremediation,
June 4-7, 2001, San Diego, CA.

Fuierer, A. M., R. S. Bowman, and T. L. Kieft. Biodegradation of toluene sorbed to a surfactant-modified zeolite.
American Society for Microbiology General Meeting, Orlando, FL, May 2001.

Brockman, F. J., D. O. Oliver, X. Yin, and T. L. Kieft. Effects of unsaturated flow on hexavalent chromium reduction
and 16s rRNA profiles. American Society for Microbiology General Meeting, Orlando, FL, May 2001.

Yin, X., D. O. Oliver, T. L. Kieft, and F. J. Brockman. Comparison of the microbial community under no flow,
unsaturated flow, and saturated flow in columns. American Society for Microbiology General Meeting, Orlando, FL,
May 2001.

McHale, M. T. L. Kieft , and N. W. Hinman. Archaeal and Bacterial diversity in a moderately acidic thermal iron
spring. NASA Astrobiology Institute meeting, Washington, DC, April, 2001.

Kieft, T. L. Vadose Zone Microbiology. Lawrence Berkeley National Laboratory/University of California, Berkekely,
CA, October 31, 2000 (Invited lecture).

McHale, M. T. L. Kieft , and N. W. Hinman. Archaeal and Bacterial diversity in a moderately acidic thermal iron
spring. American Society for Microbiology General Meeting, Los Angeles, CA, May 2000.

Kieft, T. L. Microbial activities in deep vadose zones of arid regions. American Society for Microbiology, Chicago, IL,
May 1999 (Invited lecture).

Onstott, T. C, D.P. Moser, H.Dong, J. K. Fredrickson, F.J. Brockman, T.J. Phelps, S.M. Pfiffner, A. Peacock, D.C.
White, S. Macnaughton, F.S. Colwell, D.L. Balkwill, M.F. DeFlaun, T. Kieft, D.R. Boone, A.L. Reysenbach, J. Hoek,
G.I. Omar, W.C. Ghiorse G. Southam G. Slater, B. Sherwood-Lollar, S. Kotelnikova, K. Pedersen, L. Pratt, J. Fong,
B. Baker, C. Wimpee, B. MacGregor, S. Fishbain, D.A. Stahl, M. Stute, and R. Hoover. The Witwatersrand Deep
Microbiology Project: A Window Into the Extreme Environment of Deep Subsurface Microbial Communities.
American Geophysical Union, San Francisco, December 1999.

Onstott, T. C., J. K. Fredrickson, T. J. Phelps, D. C. White, F. S. Colwell, D. L. Balkwill, M. F. DeFlaun, T. L. Kieft,
D. R. Boone, J. M. Suflita, L. R. Krumholz, and W. T. Griffin. New life in old rocks? A testimony to ten years of
subsurface microbiology. American Geophysical Union, San Francisco, December 1998.

Kieft, T. L. Microbial ecology of the vadose zone. Eighth International Symposium on Microbial Ecology. Halifax,
Nova Scotia, Canada. August 9-14, 1998. (Invited lecture).

Kieft, T. L., J. K. Fredrickson, and T. C. Onstott. Metal reduction by a Thermus sp. isolated from an ultradeep South
African gold mine. Eighth International Symposium on Microbial Ecology. Halifax, Nova Scotia, Canada. August 9-14,
1998.

Kieft, T. L., J. K. Fredrickson, T. C. Onstott, Y. A. Gorby, T. J. Bailey, D. W. Kennedy, S. W. Li, A. E. Plymale, C.
M. Spadoni, M. S. Gray. Dissimilatory Reduction of Fe(III), Mn(IV), and Other Electron Acceptors by a Thermus sp.
Isolated from an Ultradeep South African Gold Mine. American Society for Microbiology, Atlanta, GA, May 1998.

Lehman, R. M., F. S. Colwell, R. Smith, M. Delwiche, J. K. Fredrickson, T. Kieft, T. J. Phelps, and D. C. White.
Alteration of the Microbial Ecology of a Fractured Basalt Aquifer by Contaminants. American Society for
Microbiology, Atlanta, GA. May 1998.

Colwell, F., R. Smith, J. K. Fredrickson, J. P. McKinley, R. Lehman, M. Delwiche, T. McLing, T. Kieft, J. Bukowski,
and A. Fryar. Biologically active contaminant degradation zones in a basalt aquifer. American Geophysical Union, San
Francisco, CA, December 1997.

Kieft, T. L. One million years of microbial survival and succession in a sequence of buried sediments. University of
Montana, Missoula, MT, October 6, 1997. (Invited lecture).

Kieft, T. L., E. M. Murphy, P. S. Amy, D. L. Haldeman, D. B. Ringelberg. Laboratory and Field evidence for Long-
term Starvation Survival of Microorganisms in Subsurface Terrestrial Environments. SPIE Symposium: Instruments,
Methods, and Missions for the Investigation of Extraterrestrial Microorganisms, San Diego, CA, 27 July - 1 August,
1997. (Invited lecture).

Kieft, T. L., D. L. Haldeman, P. S. Amy, E. M. Murphy, B. N. Bjornstadt, E. V. McDonald, D. B. Ringelberg, D. C.
White, J. O. Stair, R. P. Griffiths, T. C. Gsell, W. E. Holben, and D. R. Boone. Microbial survival and succession in a
sequence of buried sediments during the last 1 million years. American Geophysical Union, San Francisco, CA,
December 18, 1996. (Invited lecture).

Kieft, T. L., D. L. Haldeman, P. S. Amy, E. M. Murphy, B. N. Bjornstadt, E. V. McDonald, D. B. Ringelberg, D. C.
White, J. O. Stair, R. P. Griffiths, T. C. Gsell, W. E. Holben, and D. R. Boone. Microbiology of a paleosol
chronosequence during the last 200,000 years. International Symposium on Subsurface Microbiology, Davos,
Switzerland, September 16-20, 1996.

Kieft, T. L., K. O'Connor, E. Wilch, D. B. Ringelberg and D. C. White.Long-term survival rates and phospholipid
fatty acid signature lipids of subsurface microorganisms in sediment microcosms. American Society for Microbiology
Annual Meeting, New Orleans, May 1996.

Kieft, T. L. Potential for bioremediation of contaminated subsurface environments. Facultad de Ciencias Quimiucas,
La Universidad Autonoma de Chihuahua. October 6, 1995. (Invited lecture.)

Kieft, T. L. Microbiology of the deep biosphere. Facultad de Ciencias Quimiucas, La Universidad Autonoma de
Chihuahua. October 5, 1995. (Invited lecture.)

Haldeman, D. L., P. S. Amy, T. L. Kieft, W. Spangenberg, D. Ringelberg and D. White. The microbiology of a
chronosequence of paleosols from the Channeled Scablands of arid eastern Washington. American Society for
Microbiology Annual Meeting, May 1995.

Kieft, T. L. Microbial geriatrics: long-term survival of microorganisms in the subsurface. Department of Biology,
Rensselaer Polytechnical Institute. April 1995. (Invited lecture.)

Kieft, T. L. Subsurface microbial geriatrics. Hydrology Program, New Mexico Tech. November 1994. (Invited
lecture.)

Kieft, T. L. Subsurface Microbiology. Biology Department, Texas Tech University. February 1994. (Invited lecture.)

Kieft, T. L., P. S. Amy and F. M. Phillips. Origins of subsurface microorganisms: relating laboratory microcosm
experiments to a geologic time scale. Second International Symposium on Subsurface Microbiology, Bath, England,
September 19-24, 1993.